Fig. 8. Colocalization of cannabinoid receptor 1 (CB1) with markers of primary afferents in the superficial dorsal horn of the spinal cord of sarcoma-implanted mice. ( A1 –A3 ) Colocalization of CB1 with calcitonin gene-related peptide (CGRP) ( green ) and isolectin B4 (IB4) ( blue ) in lamina I and IIo. ( B1 –B3 ) colocalization of CB1 with cholera toxin β subunit (CTb) ( green ) in lamina vIIi. Arrows = CGRP-immunoreactive (ir), IB4-positive, and CTb-ir. Scale bar = 5 μm.