Fig. 3. External signal-regulated kinase (ERK), phospho -ERK ( p -ERK), c-Jun N -terminal kinase (JNK), and phospho -JNK ( p -JNK) protein levels are unaffected by the various treatments. ( A and C ) Typical blots show expression of ERK and p -ERK ( A ) and JNK and p -JNK ( C ) protein in the spinal cord dorsal horn of the five groups (n = 4 of each group). ( B and D ) Pooled densitometric results for ERK and p -ERK ( B ) and JNK and p -JNK ( D ), with the control band intensity assigned the value of 1. No significant difference was found among the groups. β-actin was used as the loading control. CONT = saline infusion; MO = morphine infusion; MO+AMI = morphine plus amitriptyline coinfusion; MO+AMI/Anti-IL-10Ab = morphine plus amitriptyline coinfusion plus anti-IL-10 antibody injection for 5 days; MO+AMI+SB = morphine plus amitriptyline plus SB203580 coinfusion.