Fig. 1. Experimental design. Mice were subjected to breathe either air or 80 ppm hydrogen sulfide (H2S) spontaneously for 6 h or they received mechanical ventilation (vent) with 12 ml/kg for 6 h with either synthetic air or synthetic air + 80 ppm H2S. Airway pressure, blood pressure, and body temperature were continuously measured and noted every 30 min. Blood gas analyses were performed at the end of the experiment and in ventilated groups in addition to after the onset of ventilation. Bronchoalveolar lavage (BAL) and lung tissue were analyzed for the parameters depicted in the “analysis” box. TUNEL = terminal deoxynucleotide transferase-mediated dUTP-biotin nick-end labeling; VILI = ventilator-induced lung injury.
