Fig. 9. Effect of body temperature on lung injury after ventilation and hydrogen sulfide (H2S) treatment. Mice were ventilated (vent) for 6 h either with synthetic air at a mean body temperature (Tb) of 34°C (B ) or 36°C (C ) or with synthetic air + 80 ppm H2S at a mean body temperature of 34°C (D ) or 36°C (E ). As controls, mice were subjected to spontaneously breathe air (A ). Left lung lobes were harvested, fixed, cut, and hematoxylin and eosin stained. Representative pictures are shown for each experimental group (magnification ×400). High-power fields were randomly assigned per photograph, and alveolar wall thickness (F ) and ventilator-induced lung injury (VILI) score (G ) were determined. All graphs represent means ± SEM, n = 6/group. Analysis of variance (Student-Newman-Keuls post hoc test), *P < 0.05 versus nonventilated control; §P < 0.05 versus hypothermic air-ventilated groups; ‡P < 0.05 versus H2S-ventilated groups.