Fig. 4.  Midazolam inhibits the production of tumor necrosis factor-α (TNF-α) and interferon-γ (IFN-γ) from CD4-positive T cells stimulated by allogeneic dendritic cell culture. Mixed cell culture was performed for 4 days with bone marrow–derived dendritic cells from C57BL/6 mice (haplotype I-Ab) and CD3-positive T cells from Balb/c mice (haplotype I-Ad). On day 4, T cells were harvested and restimulated with ionomycin and phorbol myristate acetate in the presence of BD GolgiStop. Production of TNF-α, IFN-γ and interleukin-4 (IL-4) from CD4-positive T cells was assessed by flow cytometry with intracellular staining of cytokine. Results are presented as mean ± SD (N = 8). (A ) Typical flow-cytometry diagram depicting percentage of mean fluorescence intensity. (B ) *P < 0.05, control versus  midazolam. BM-DC = bone marrow derived dendritic cell; DC = dendritic cell; IFN-α= interferon-α; TNF-α= tumor necrosis factor-α.