Fig. 5. Midazolam (MDZ) inhibits T helper 1–type immune response in dendritic cell (DC)–transfer model of contact hypersensitivity. After culture in the presence (or absence) of midazolam, 5 × 105DCs were pulsed with 100 μg/ml 2,4-dinitrobenzene sulfonic acid and injected subcutaneously on day 0. After 5 days, mice were challenged with application of 10 μl 2,4-dinitro-1-fluorobenzene, 0.2%, in 4:1 acetone/olive oil solution to both sides of right ear. Right (challenged) and left (unchallenged) ear thickness was measured after 24 h. Results, which are presented as mean ± SD (N = 8), are shown as simple subtraction. In control groups, DCs were either not 2,4-dinitrobenzene sulfonic acid–pulsed (negative [−] control) and injected subcutaneously or animals (positive [+] control) were shaved and abdominal skin was painted with 2,4-dinitro-1-fluorobenzene, 0.5% (w/v). After 5 days, ear thickness was challenged epicutaneously with 2,4-dinitro-1-fluorobenzene, 0.2%, and ear thickness was measured after 24 h. *P < 0.05, phosphate buffer saline (PBS)–DC versus MDZ-DC or positive (+) control versus negative (−) control.