Fig. 6. Inhibition related to glycine concentration. (A ) Cyclopropane inhibition of wild-type GluN1/2A receptors is concentration dependent. The curve is fitted to a Hill equation. The points are mean values of 6–14 cells at each cyclopropane concentration. (B ) Cyclopropane (10%) inhibition of wild-type GluN1/2A receptors is not glycine dependent. The points are mean values of an average of 13 cells at each glycine concentration. (C ) Inhibition of wild-type GluN1/2A receptors by N2O (80%) is glycine dependent. (D ) Inhibition of GluN1(F758W)/2A receptors by N2O (80%) is glycine dependent. (E ) Inhibition of GluN1(F758Y)/2A receptors by N2O (80%) is not glycine dependent. (F ) Inhibition of GluN1 (F758Y)/2A receptors by xenon (80%) is not glycine dependent. The % of control represents the ratio of the current in the presence of anesthetic to that in the absence of anesthetic at each glycine concentration. The points are mean values of an average of eight cells at each glycine concentration. *P < 0.025 is significantly different from inhibition at 100 μM glycine. Cyclo = cyclopropane. N2O = nitrous oxide.