Fig. 7. Representative light microphotographs showing the presence of mast cells after the sham or surgical procedure in the paw tissue of mice. A section was obtained from the paw tissue 10 min after sham (A , B ) or surgical (C , D ) procedure. Percentage of degranulated mast cells relative to intact mast cell (E ). The vertical bars  represent the means + SEM (n = 5 to sham-operated group and n =6 to operated groups). ***P < 0.001 when compared to the sham group; Student t  test.