Fig. 3. Anesthesia with 2.5% sevoflurane for 2 h in pregnant mice at G14 decreases synaptophysin levels in the hippocampus of offspring mice examined at P31. (A ) Sevoflurane anesthesia decreases synaptophysin levels in the brain tissues of offspring mice as compared with the control condition on immunohistochemistry analysis. (B ) Quantification of the immunohistochemistry image shows that sevoflurane anesthesia decreases synaptophysin levels in the mouse brain tissues as compared with the control condition (**P = 0.0003) (n = 6 per arm).