Regional analysis of γ-aminobutyric acid type A (GABA_A) plus neurons in the thoracic spinal cord. (A) Representative image of spinal cord regions of interest; superficial laminae (I to II), deep laminae (III to VII, X), and intermediolateral nucleus. Magnification is 4×, and scale bar is 100 µm. (B) Percentage of GABA_A plus neurons (expressing neuronal marker neuronal nuclei) in each regional area for ischemia–reperfusion versus plus spinal cord stimulation. Data displayed as mean ± SD. *P < 0.05 for regional comparison of ischemia–reperfusion versus ischemia–reperfusion plus spinal cord stimulation. Control (n = 5). Ischemia–reperfusion (n = 5). Ischemia–reperfusion plus spinal cord stimulation (n = 5).