Fig. 5.
Decreased diversity of hippocampal cornu ammonis 1 pyramidal neurons induced by sevoflurane exposures in male and female mice. (A) The t-distributed stochastic neighbor embedding plots showing 19 cornu ammonis 1 pyramidal neuron subclusters in the control male group (left) and sevoflurane male group (right). (B) The percentage of cells in specific cornu ammonis 1 neuron subclusters in the control male and sevoflurane male groups. (C) Differentiation states of cornu ammonis 1 pyramidal neuron in two dimensions in the control male group (left) and sevoflurane male group (right). (D) Expression pattern of the top genes (Cacnb2, Cst3, Dpp10, Lingo2, Lrrc4c, and Tshz2) in the control male group (upper) and sevoflurane male group (lower). n = 15,821 cells (control male) and n = 3,154 cells (sevoflurane male). Criteria for top genes: false discovery rate less than 1e-5 (Monocle 2 package). (E) The t-distributed stochastic neighbor embedding plots showing 14 cornu ammonis 1 pyramidal neuron subclusters in the control female group (left) and sevoflurane female group (right). (F) The percentage of cells in specific cornu ammonis 1 neuron subclusters in the control female and sevoflurane female groups. (G) Differentiation states of cornu ammonis 1 neurons in the control female group (left) and sevoflurane female group (right). (H) Expression pattern of the top genes (1700007F19Rik, 4921539H07Rik, A830018L16Rik, Brinp3, Dpp10, and Lrrc4c) in the control female group (upper) and sevoflurane female group (lower). Criteria for top genes: false discovery rate less than 1e-5 (Monocle 2 package). n = 5,188. cells (control female) and n = 4520 cells (sevoflurane female).