Differentially expressed genes induced by sevoflurane exposures in hippocampal cell types in male mice. (A) Histogram showing the number of differentially expressed genes in hippocampal cell types, with plots (red) indicating differentially expressed genes unique to a cell type and plots and lines (black) indicating common differentially expressed genes between two or more cell types. (B) Histogram with plots and lines showing the unique and common differentially expressed genes in hippocampal neuron subtypes. (C) Heatmaps of differentially expressed genes unique to specific cell types (endothelial cells, neurons, astrocytes, cornu ammonis 3 neurons, and cornu ammonis 1 neurons) showing differential expression patterns between the control male group (blue) and sevoflurane male group (red). (D) Common differentially expressed genes captured between the whole-tissue analysis and single-cell genomic analysis. Criteria for differentially expressed genes: mincell_pct 0.25 or greater, q value ≤ 0.05, and |log2(fold change)| 0.585 or greater (Model-based Analysis of Single-cell Transcriptomics test).