Fig. 3. Relative fluorescence intensity of sparged and unsparged blood samples with no surfactant, Dow Corning Antifoam 1510US (DCA), Perftoran, or Pluronic F-127 (PF-127) added (n = 6 per group). Shear conditions were as follows: (A ) 100 s⁻¹for 5 min; (B ) 100 s⁻¹for 10 min; (C ) 100 s⁻¹for 20 min; (D ) 500 s⁻¹for 5 min; (E ) 500 s⁻¹for 10 min; and (F ) 500 s⁻¹for 20 min. Baseline fluorescence intensity was the value measured without surfactant for samples sheared at 100 s⁻¹for 5 min but not sparged. *P < 0.05 compared with the not sparged group; #P < 0.05 compared with the sparged and no surfactant group.