Fig. 1. ( A ) Endothelial nitric oxide synthase (eNOS) and inducible nitric oxide synthase (iNOS) protein levels in whole kidney homogenate after ischemia–reperfusion were visualized by immunoblotting with antibodies against eNOS and iNOS. ( B and C ) Densitometric analysis was performed after normalization with actin. Blots shown are representative of Western blot analysis from four separate experiments (* P < 0.05 vs. sham-operated animal).