Fig. 5. (  A ) Dimethylarginine dimethylaminohydrolase-1 (DDAH-1) and dimethylarginine dimethylaminohydrolase-2 (DDAH-2) protein levels in whole kidney homogenate after ischemia–reperfusion were visualized by immunoblotting with antibodies against DDAH-1 and DDAH-2. (  B and  C ) Densitometric analysis was performed after normalization with actin. Blots shown are representative of Western blot analysis from four separate experiments (*  P < 0.05  vs. sham-operated animal).