Fig. 4. Representative images of microglia in the ipsilateral (ipsi) and contralateral (contra) spinal cord after paw incision. Microglia are represented by OX42 immunoreactivity after twice-daily intraperitoneal injection of vehicle ( A , B , and C ), 50 mg · kg−1· day−1( D , E , and F ) and 100 mg · kg−1· day−1( G , H , and I ) minocycline for 3 days. ( A ), ( D ) and ( G ) are high-magnification images of the squares in the ipsilateral spinal cord shown in ( B ), ( E ) and ( H ), respectively. Similarly, ( C ), ( F ), and ( I ) are high-magnification images of the squares in the contralateral spinal cord shown in ( B ), ( E ) and ( H ), respectively. Increased OX42 expression was observed in the medial parts of the ipsilateral dorsal horn of the spinal cord after injection of vehicle. Minocycline administration reduced the upregulation of OX42 immunoreactivity after paw incision. Scale bar = 400 μm.
