Fig. 2. (A ) Effects of dexmedetomidine (Dex: 10−6m, 30 min of application) postconditioning on cell death labeled by propidium iodide (PI) fluorescence induced by different reperfusion times (10, 30, 60, 90, and 120 min) after oxygen and glucose deprivation (OGD) in CA1 of mouse organotypic slices cultures. Data (mean ± SD) are expressed as an increase in fluorescence intensity from control (control: 100%). ANOVA for PI fluorescence were F = 214.6, P < 0.0001. Post hoc analysis used Bonferroni correction. ***P < 0.001 versus control. (B ) Effects of increasing dexmedetomidine concentrations (10−8to 10−4m) on postconditioning. Data (mean ± SD) are expressed as fluorescence intensity increase from control (control: 100%). ANOVA for PI fluorescence was F = 11.07, P < 0.0001. Post hoc analysis used Bonferroni correction. *P < 0.05; ***P < 0.001 versus control. NS = nonsignificant.
