Fig. 4. Western blot analysis of myocardial expression of Pim-1 kinase. Results are presented as representative original immunoblottings and average densitometric results as percentage of control (n = 6 per group). CON = control group; DES = desflurane (1.0 minimum alveolar concentration); DES + PIM-Inh.II = Pim-1 kinase inhibitor II in combination with desflurane; IPC = ischemic preconditioning (three cycles of 5 min ischemia–reperfusion each); IPC + PIM-Inh.II = Pim-1 kinase inhibitor II in combination with ischemic preconditioning; PIM-Inh.II = Pim-1 kinase inhibitor II (10 μg/g).