Fig. 1. Schematic representation of the study design. Each animal underwent two experiments separated by 5–7 days. The first was to examine the response to multiple submaximal doses of CW002 for estimation of the dose required to produce 95% neuromuscular blockade (ED⁹⁵) and to confirm cysteine reversal. The second experiment was designed to characterize histamine release and cardiopulmonary effects of incrementally larger doses of CW002 administered as multiples of the ED⁹⁵. TOF = train-of-four stimulation.