Fig. 5.  Gs (stimulatory G-protein) inhibitors blocked facilitatory effect of dexmedetomidine (Dex) on potassium chloride (KCl)-evoked acetylcholine release from spinal nerve ligation (SNL) synaptosomes. Data are presented as percentage of baseline release. SNL synaptosomes were prepared at 14 days after surgery and treated with 12 mm KCl or a combination of 12 mm KCl with 10 nm Dex in the presence or absence of suramin (10 μm) or NF449 (10 μm). Suramin and NF449 were present throughout the perfusion. *P < 0.05 versus  KCL alone and # P < 0.05 versus  Dex alone by one-way analysis of variance.