Fig. 2. Isoflurane induced greater apoptosis than equipotent exposure of sevoflurane in the hippocampus CA1 region of neonatal developing brains. (A ) Brain sections containing hippocampus from various experimental groups were immunostained to determine caspase-3 positive cells, and the sample sections were illustrated at different magnifications. Arrows  indicate caspase-3 positive cells in the hippocampus CA1 region located within the rectangles  illustrated on the left  panel demonstrating whole hippocampus of neonatal mice developing brains at low magnification. Scale bar is in the right low corner of each brain section and is 50 μm for the low magnification on the left panel  and 10 μm for the high magnification on the right panel  of brain sections. (B ) Statistical analysis of caspase-3 positive cell from various experimental groups. Data represent mean ± SE. N = 12, 12, and 9 for control, isoflurane (Iso), and sevoflurane (Sevo) groups, respectively. +++P < 0.001 compared with control. **P < 0.01 compared with sevoflurane.