Fig. 7. Intrathecal (i.t.) pre- or posttreatment with EphB1-Fc inhibited chronic constrictive injury (CCI)-induced neuropathic pain and spinal Fos protein expression, accompanied by the decreased expression of spinal phosphorylated mitogen-activated protein kinases (p-MAPKs). (A ) Schematic illustration of pretreatment experimental protocol. (B ) Schematic illustration of posttreatment experimental protocol. (C ) Pretreatment of EphB1-Fc (0.5 μg) prevented CCI-induced thermal hyperalgesia in paw-withdrawal latency (PWL) test and mechanical allodynia in paw-withdrawal threshold (PWT) test. *P < 0.05, **P < 0.01, compared with EphB1-Fc + CCI, n = 10 mice in each group. (D ) The representative immunohistochemical staining and the quantitative data for the decreased CCI-induced spinal Fos expression by pretreatment with EphB1-Fc. **P < 0.01 compared with saline (Sal) + CCI group, n = 6 mice in each group, Scale bar = 100-μm. (E ) The representative bands and the quantitative data for the decreased expression of spinal p-p38, p-ERK, and p-JNK by CCI after pretreatment with EphB1-Fc. The fold change for the density of each p-MAPKs normalized to total MAPKs for each sample. The fold change of p-MAPKs levels in Sal + Sham group were set at 1 for quantifications. **P < 0.01 or *P < 0.05 compared with Sal + Sham group, # P < 0.05 compared with Sal + CCI group, n = 4 mice in each group. (F ) Posttreatment of EphB1-Fc reversed CCI-induced thermal hyperalgesia and mechanical allodynia. *P < 0.05, compared with CCI + EphB1-Fc, n = 10 mice in each group. (G ) The representative immunohistochemical staining and the quantitative data for the reversed CCI-induced spinal Fos expression by posttreatment with EphB1-Fc. **P < 0.01 compared with CCI + Sal group, n = 6 mice in each group, Scale bar = 100-μm. (H ) The representative bands and the quantitative data for the decreased expression of spinal p-p38, p-ERK, and p-JNK by CCI after posttreatment with EphB1-Fc. The fold change for the density of each p-MAPKs normalized to total MAPKs for each sample. The fold change of p-MAPKs levels in Sal + Sham group were set at 1 for quantifications. *P < 0.05 compared with Sham + Sal group, # P < 0.05 compared with CCI + Sal group, n = 4 mice in each group.

Fig. 7. Intrathecal (i.t.) pre- or posttreatment with EphB1-Fc inhibited chronic constrictive injury (CCI)-induced neuropathic pain and spinal Fos protein expression, accompanied by the decreased expression of spinal phosphorylated mitogen-activated protein kinases (p-MAPKs). (A ) Schematic illustration of pretreatment experimental protocol. (B ) Schematic illustration of posttreatment experimental protocol. (C ) Pretreatment of EphB1-Fc (0.5 μg) prevented CCI-induced thermal hyperalgesia in paw-withdrawal latency (PWL) test and mechanical allodynia in paw-withdrawal threshold (PWT) test. *P < 0.05, **P < 0.01, compared with EphB1-Fc + CCI, n = 10 mice in each group. (D ) The representative immunohistochemical staining and the quantitative data for the decreased CCI-induced spinal Fos expression by pretreatment with EphB1-Fc. **P < 0.01 compared with saline (Sal) + CCI group, n = 6 mice in each group, Scale bar = 100-μm. (E ) The representative bands and the quantitative data for the decreased expression of spinal p-p38, p-ERK, and p-JNK by CCI after pretreatment with EphB1-Fc. The fold change for the density of each p-MAPKs normalized to total MAPKs for each sample. The fold change of p-MAPKs levels in Sal + Sham group were set at 1 for quantifications. **P < 0.01 or *P < 0.05 compared with Sal + Sham group, # P < 0.05 compared with Sal + CCI group, n = 4 mice in each group. (F ) Posttreatment of EphB1-Fc reversed CCI-induced thermal hyperalgesia and mechanical allodynia. *P < 0.05, compared with CCI + EphB1-Fc, n = 10 mice in each group. (G ) The representative immunohistochemical staining and the quantitative data for the reversed CCI-induced spinal Fos expression by posttreatment with EphB1-Fc. **P < 0.01 compared with CCI + Sal group, n = 6 mice in each group, Scale bar = 100-μm. (H ) The representative bands and the quantitative data for the decreased expression of spinal p-p38, p-ERK, and p-JNK by CCI after posttreatment with EphB1-Fc. The fold change for the density of each p-MAPKs normalized to total MAPKs for each sample. The fold change of p-MAPKs levels in Sal + Sham group were set at 1 for quantifications. *P < 0.05 compared with Sham + Sal group, # P < 0.05 compared with CCI + Sal group, n = 4 mice in each group.

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