Fig. 8. Intrathecal (i.t.) pretreatment with EphB1-Fc inhibited formalin-induced inflammation pain and spinal Fos protein expression, accompanied by the decreased expression of spinal phosphorylated mitogen-activated protein kinases (p-MAPKs). (A ) Schematic illustration of experimental protocol. (B ) Pretreatment of EphBs receptors blocker EphB1-Fc (0.5 μg) decreased the time of licking/biting paw induced by formalin. *P < 0.05 or **P < 0.01 compared with saline (Sal)-Formalin group, n = 8 mice in each group. (C ) The representative immunohistochemical staining and the quantitative data for the decreased formalin-induced Fos expression by pretreatment with EphB1-Fc (0.5 μg), not saline, in the spinal cord of mice. **P < 0.01 compared with Sal + formalin group, n = 6 mice in each group, scale bar = 100 μm. (D ) The representative bands and the quantitative data for the decreased expression of spinal p-p38, p-ERK, and p-JNK by injection of formalin after pretreatment with EphB1-Fc (0.5 μg). The fold change for the density of each p-MAPKs normalized to total MAPKs for each sample. The fold change of p-MAPKs levels in Sal + Sal group was set at 1 for quantifications. *P < 0.05 compared with Sal + Sal group, # P < 0.05 compared with Sal + formalin group n = 4 mice in each group.