Fig. 4. Anesthesia with 3% sevoflurane for 6 h induces a greater degree of caspase-3 activation in the brain tissues of neonatal Alzheimer disease (AD) transgenic mice than that in neonatal naïve mice. (A ) Anesthesia with sevoflurane anesthesia (lanes 2 and 4) induces caspase-3 activation when compared with the control condition (lanes 1 and 3) in naïve mice and AD transgenic mice, respectively. Sevoflurane anesthesia induces a greater degree of caspase-3 activation in AD transgenic mice (lane 4) than in naïve mice (lane 2). (B ) Quantification of the Western blot shows that sevoflurane anesthesia (black bar  and hatched bar ) induces caspase-3 activation compared with the control condition (white bar  and gray bar ) in both neonatal naïve and AD transgenic mice, respectively. Sevoflurane anesthesia induces a greater degree of caspase activation in AD transgenic mice (hatched bar ) than in naïve mice (black bar ). We have averaged the results from four independent experiments. FL = full length. **P < 0.01; ## P < 0.01.