Fig. 6. Anesthesia with 3% sevoflurane for 6 h increases Aβ levels in the brain tissues of neonatal naïve and Alzheimer disease (AD) transgenic mice. (A ) Sevoflurane anesthesia (lanes 3 and 4 and lanes 7 and 8) increases Aβ levels when compared with the control condition (lanes 1 and 2 and lanes 5 and 6) in the brain tissues of neonatal naïve and AD transgenic mice, respectively. (B ) Quantification of the Western blot shows that sevoflurane anesthesia increases Aβ levels (black bar  and hatched bar ) when compared with the control condition (white bar  and gray bar ) in the brain tissues of neonatal naïve mice and AD transgenic mice, respectively. (C ) Sandwich enzyme-linked immunosorbent assay (ELISA) shows that sevoflurane anesthesia increases Aβ42 levels in the brain tissues of AD transgenic mice. (D ) ELISA sandwich shows that sevoflurane anesthesia does not increase Aβ40 levels in the brain tissues of AD transgenic mice. We have averaged the results from six independent experiments. Aβ=β-amyloid protein; N.S. = not significant. *P < 0.05; **P < 0.01; ## P < 0.01.