Fig. 1. Time course of mechanical hypersensitivity induced by paw incision after intrathecal treatment with anti-chemokine (C-C motif) ligand 2 (CCL2) immunoglobulin G (IgG) (3 ng, dotted bars ; 10 ng, gray bars ) or control IgG (10 ng, open bars ) was examined acutely for several minutes after treatment beginning 1 day after surgical incision (A ) and for several days after single treatment beginning 1 day after surgical incision (B ). Withdrawal thresholds to von Frey filaments values are expressed as the median (solid line ) and 25th and 75th percentiles of 8–14 rats per group. *P < 0.05 for comparisons to control IgG values at each time point or # P < 0.05 for comparison between 3 ng anti-CCL2– and 10 ng anti-CCL2–treated values at each time point by Kruskal-Wallis analysis of variance on ranks followed by Dunn post hoc  test. Black arrowhead  indicates time point of intrathecal injection.