Fig. 3. Quantification of ionized calcium-binding adapter molecule 1 (IBA1) immunostaining in ipsilateral (ipsi) (gray bar ) and contralateral (contra) (open bar ) dorsal spinal cord of sham-operated and incision rats 30 min after intrathecal administration of anti-chemokine (C-C motif) ligand 2 (CCL2) (10 ng) or control immunoglobulin G (IgG) (10 ng) on day 1 postoperatively (1 dpo; A ) or 24 h after administration of anti-CCL2 (10 ng) or control IgG (10 ng) on day 2 postoperatively (2 dpo; B ). *P < 0.05 compared with sham + control IgG value within side. # P < 0.05 compared with contralateral side within treatment group. +P < 0.05 compared with incision control IgG-treated values by two-way analysis of variance with Bonferroni post hoc  test (n = 8 rats per group).