Fig. 1. The cortical cells in culture are primarily neurons. The cortical cells were dissociated and cultured for 3 days before fixation and immunohistochemistry. These cells were imaged with fluorescence microscopy. (A ) Neurons were identified with Tuj1 antibody (red ). (B ) Nuclear-specific 4′-6-diamidino-2-phenylindole stain (blue ) labeled all cells. (C ) Merged image: counting revealed that 90% of cultured cells are neurons (pink ; scale bar = 200 μm).