Fig. 2. (A ) Representative trace depicting the effect of a single application of capsaicin (Cap; 100 nM), the specific transient receptor potential ankyrin subtype-1 (TRPA1) activator allyl isothiocyanate (AITC; 100 μM), or propofol (Pro; 10 μM) on intracellular free calcium concentration in mouse dorsal root ganglion (DRG) neurons. (B through D ) Representative traces depicting the effect of time, Pro (10 μM), and AITC (100 μM), respectively, after capsaicin-induced (100 nM) desensitization on restoration of transient receptor potential vanilloid receptor subtype-1 (TRPV1) sensitivity in mouse DRG neurons that contain only functional TRPV1 receptors. (E ) Summarized data for B through D . Data are expressed as a percentage of the response to the final application of capsaicin (Final Capsaicin) in the untreated control and reported as the mean ± SD. (The DRG neurons were obtained from five different mice.)