Fig. 5.  Involvement of PI3K-Akt and ERK pathways and their downstream target GSK-3β in Intralipid -induced protection. (A , C , E ) Representative immunoblots of pAkt and total Akt (A ), phospho (p)ERK1,2 and total ERK1,2 (C ), and pGSK-3β and total GSK-3β (E ) in heart homogenates subjected to ischemia/reperfusion from the control group (CTRL), Intralipid group (ILP), and 1% Intralipid+LY294002 (ILP+LY), top panels, or CTRL, ILP, and 1% Intralipid+PD98059 (ILP+PD), bottom panels. (B , D , F ) Western blot quantification (mean±SEM) of pAkt protein to total Akt (B ), pERK1 to total ERK (D ), and pGSK-3β to total GSK-3β (E ) ratios in CTRL (white bars , n = 6), ILP (black bars , n = 4), ILP+LY (dark gray bars , n = 4), and ILP+PD (light gray bars , n = 4). **P < 0.01 versus  CTRL; ## P < 0.01 versus  ILP.

Fig. 5.  Involvement of PI3K-Akt and ERK pathways and their downstream target GSK-3β in Intralipid -induced protection. (A , C , E ) Representative immunoblots of pAkt and total Akt (A ), phospho (p)ERK1,2 and total ERK1,2 (C ), and pGSK-3β and total GSK-3β (E ) in heart homogenates subjected to ischemia/reperfusion from the control group (CTRL), Intralipid group (ILP), and 1% Intralipid+LY294002 (ILP+LY), top panels, or CTRL, ILP, and 1% Intralipid+PD98059 (ILP+PD), bottom panels. (B , D , F ) Western blot quantification (mean±SEM) of pAkt protein to total Akt (B ), pERK1 to total ERK (D ), and pGSK-3β to total GSK-3β (E ) ratios in CTRL (white bars , n = 6), ILP (black bars , n = 4), ILP+LY (dark gray bars , n = 4), and ILP+PD (light gray bars , n = 4). **P < 0.01 versus  CTRL; ## P < 0.01 versus  ILP.

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