Fig. 7. Effect of MyD88 and Trif deficiency on endotoxin-induced cytokine production in serum. WT, MyD88^−/−, and Trif^−/−mice were administered LPS (15 mg/kg, intraperitoneal injection) or saline. Six hours later, blood was collected. Serum IL-6, TNFα, and IL-1β were measured with a multiplex fluorescent bead-based immunoassay. Each error bar represents mean ± SE. The number of animals in each group: WT-Saline = 5, WT-LPS = 7, MyD88^−/−-Saline = 5, MyD88^−/−-LPS = 5, Trif^−/−-Saline = 3, Trif^−/−-LPS = 6. *P < 0.05; **P < 0.01; ***P < 0.001. IL = interleukin; LPS = lipopolysaccharide; MyD88 = myeloid differentiation factor 88; TNF = tumor necrosis factor; Trif = TIR-domain-containing adaptor protein inducing interferon-β mediated transcription-factor; WT = wild-type.