Fig. 4. Effect of intra-ACC injection of U0126 or PD98059 on ERK phosphorylation in ACC. (A ) A representative image of Nissl staining in the ACC after drug injection. The brown staining in the ACC shows the injection site. Bar, 500 μm. (B ) Representative Western blot of p-ERK and total ERK in the ACC after drug injection. (C ) Semiquantitative analysis of p-ERK and t-ERK normalized to GAPDH after drug treatment (n = 4 for each group). ACC = anterior cingulate cortex; GAPDH = glyceraldehyde-3-phosphate dehydrogenase; p-ERK = phosphorylated extracellular signal-regulated kinase; t-ERK = total ERK. **P < 0.01 versus  the vehicle treatment. One-way ANOVA followed by Dunnett post hoc  test (C  and D ).