Fig. 6. Effect of U0126 on mechanical nociceptive threshold and anxiety-like behavior 6 h after incision. U0126 was administered through intra-ACC injection. One hour after intra-ACC injection of U0126, PWT (A ), EPM (B ), and open-field test (C  and D ) were examined. The decreased PWT induced by hind paw incision is not attenuated by U0126 treatment (A ). However, EPM and open field test show that time spent in open arm or in the inner area are greatly longer in the U0126-treated groups (n = 6) compared with the vehicle-treated group (n = 6). The total travel distance is not significantly different between vehicle-treated rats and U0126-treated groups. ACC = anterior cingulate cortex; PWT = paw withdrawal threshold; EPM = elevated plus maze. *P < 0.05 versus  the vehicle treatment; **P < 0.01 versus  the baseline. Two-way ANOVA followed by Tukey post hoc  test (A ), unpaired two-tailed Student t  test (B–D ).