Fig. 2. Primary neurons were isolated from neonatal rodent brains at postnatal day 1–3 and grown in vitro for 5–7 days. On day in vitro 7 neurons were exposed to propofol 3.0 μM for 6 h in 5% CO2in air. Neuritic processes were evaluated after propofol exposure by drebrin immunofluorescence microscopy. Nucleus was stained with DAPI (4′,6-diamidino-2-phenylindole). (A ) Immunofluorescence microscopy shows a decrease in drebrin in neurons exposed to propofol versus control. (B ) Quantitation of the data are represented in the graph (n = 5; #P = 0.008). Sample size is indicated above the error bars ± SEM. These data demonstrate that, in vitro , primary neurons from neonatal rodents exposed to propofol exhibit a reduction in neuritic processes. DAPI = 4′,6- diamidino-2-phenylindole.