Fig. 3. Primary neurons were isolated from neonatal rodent brains at postnatal day 1–3 and grown in vitro for 5–7 days. Neurons were pretreated with TAT-Pep5 (10 μM; 15 min), a p75NTRintracellular domain antagonist, before propofol exposure. After pretreatment, neurons were exposed to propofol 3.0 μM for 6 h in 5% CO2in air. Apoptosis was evaluated after propofol exposure by cleaved caspase-3 immunoblot. (A ) Immunoblot analysis shows a decrease in apoptosis marker, cleaved caspase-3, with TAT-Pep5 pretreatment. (B ) Quantitation of the data are represented in the graph (n = 3; #P = 0.046). Sample size is indicated above the error bars ± SEM. Cl-Csp3 = cleaved caspase-3; T-Csp3 = total caspase-3.