Fig. 4. The effects of chronic fenobam injection on spontaneous formalin behavior. Mice that were administered either fenobam or vehicle for 5 days (A–C ) before the formalin test demonstrated significantly decreased time spent licking or lifting the injected paw when administered fenobam (30 mg/kg) 5 min before intraplantar formalin injection compared with mice treated with vehicle for 5 days and vehicle on the day of the experiment (two-way ANOVA main effect of treatment P < 0.0001; Bonferroni correction posttest **P < 0.01 compared with 5-day Veh/Veh). Both the first phase (B ) and the second phase (C ) were reduced (one-way ANOVA main effect of treatment P = 0.0097, P = 0.0069, respectively; Dunnett posttest *, **P < 0.05 and 0.01, respectively compared with 5-day Veh/Veh mice, n = 6 in Veh/Veh per group and n = 7 in Veh/Fen and Fen/Fen groups, respectively). Mice that were administered either fenobam or vehicle for 14 days (D–F ) before the formalin test demonstrated significantly decreased time spent licking or lifting the injected paw when administered fenobam (30 mg/kg) 5 min before intraplantar formalin injection compared with mice treated with vehicle for 14 days and vehicle on the day of the experiment (two-way ANOVA main effect of treatment P < 0.0001; Bonferroni correction posttest **P < 0.01 compared with 14-day Veh/Veh). The second phase (F ) was reduced in both groups compared to Veh/Veh mice (one-way ANOVA main effect of treatment P = 0.0154; Dunnett posttest *P < 0.05) as compared to 14-day Veh/Veh mice. The first phase (E ) was significantly reduced in vehicle/fenobam mice when compared with Veh/Veh mice (one-way ANOVA main effect of treatment P = 0.0258; Dunnett posttest *P < 0.05 compared with 14-day Veh/Veh mice). (n = 8 in the Veh/Veh and n = 11 in the Veh/Fen and Fen/Fen groups, respectively). Fen = fenobam; Veh = vehicle.