Fig. 4. Effects of Ro27-3225 treatment on expression of target genes evaluated using real-time polymerase chain reaction in rat livers. Ro27-3225 treatment significantly induced expression of (A ) nuclear receptor subfamily 4, group A, member 1 and (B ) tight junction protein 1 in livers of hemorrhage rats. Expression remained at baseline values in untreated hemorrhage animals. Bars  denote mean ± SEM (n = 6). **P < 0.01, ***P < 0.001. Nr4a1 = nuclear receptor subfamily 4, group A, member 1; Tjp1 = tight junction protein 1.