Fig. 5. Western blot analysis showing the levels of OX-42 in the ipsilateral cuneate nucleus (CN) 7 days after chronic constriction injury (CCI) in rats treated with regional or whole-body hypothermia. (A ) OX-42 levels significantly decreased after applying regional hypothermia (P < 0.05, by two-way ANOVA). There was a significant decrease in OX-42 levels in rats pretreated with mild or deep regional hypothermia, compared with those pretreated with regional normothermia (*P < 0.05, by Tukey test). Similarly, in the 5 h postinjury group, OX-42 levels in the CN were significantly decreased in CCI rats that received mild or deep regional hypothermia compared with those that received regional normothermia (*P < 0.05, by Tukey test). Furthermore, when administered preinjury and 5 h postinjury, deep regional hypothermia was more effective in suppressing OX-42 levels than mild regional hypothermia (#P < 0.05, by Tukey test). However, in 1 day, 3 days, and 5 days postinjury groups, the levels of OX-42 in the CN did not differ significantly among rats that received regional normothermia, mild regional hypothermia, or deep regional hypothermia (P > 0.05, by Tukey test). (B ) A significant decrease in OX-42 levels was noted after applying whole-body hypothermia (P < 0.05, by two-way ANOVA). Compared with whole-body normothermia, mild and deep whole-body hypothermia administered preinjury, 5 h, 1 day, and 3 days postinjury were effective in decreasing OX-42 levels in the CN of CCI rats (*P < 0.05, by Tukey test). On day 5 postinjury, a significant decrease in OX-42 levels was observed in CCI rats treated with deep whole-body hypothermia, but not mild whole-body hypothermia, compared with those treated with whole-body normothermia (*P < 0.05, by Tukey test). In addition, lower levels of OX-42 were observed in rats treated with deep whole-body hypothermia than in those treated with mild whole-body hypothermia (#P < 0.05, by Tukey test). Regional and whole-body normothermia resulted in a similar increase in OX-42 levels (P > 0.05, by two-way ANOVA). Whole-body hypothermia, either mild or deep, contributed to lower OX-42 levels than the corresponding regional hypothermia (P < 0.05, by two-way ANOVA). β-actin was used as a loading control. Error bars  represent mean ± SD; n = 5 rats per treatment.

Fig. 5. Western blot analysis showing the levels of OX-42 in the ipsilateral cuneate nucleus (CN) 7 days after chronic constriction injury (CCI) in rats treated with regional or whole-body hypothermia. (A ) OX-42 levels significantly decreased after applying regional hypothermia (P < 0.05, by two-way ANOVA). There was a significant decrease in OX-42 levels in rats pretreated with mild or deep regional hypothermia, compared with those pretreated with regional normothermia (*P < 0.05, by Tukey test). Similarly, in the 5 h postinjury group, OX-42 levels in the CN were significantly decreased in CCI rats that received mild or deep regional hypothermia compared with those that received regional normothermia (*P < 0.05, by Tukey test). Furthermore, when administered preinjury and 5 h postinjury, deep regional hypothermia was more effective in suppressing OX-42 levels than mild regional hypothermia (#P < 0.05, by Tukey test). However, in 1 day, 3 days, and 5 days postinjury groups, the levels of OX-42 in the CN did not differ significantly among rats that received regional normothermia, mild regional hypothermia, or deep regional hypothermia (P > 0.05, by Tukey test). (B ) A significant decrease in OX-42 levels was noted after applying whole-body hypothermia (P < 0.05, by two-way ANOVA). Compared with whole-body normothermia, mild and deep whole-body hypothermia administered preinjury, 5 h, 1 day, and 3 days postinjury were effective in decreasing OX-42 levels in the CN of CCI rats (*P < 0.05, by Tukey test). On day 5 postinjury, a significant decrease in OX-42 levels was observed in CCI rats treated with deep whole-body hypothermia, but not mild whole-body hypothermia, compared with those treated with whole-body normothermia (*P < 0.05, by Tukey test). In addition, lower levels of OX-42 were observed in rats treated with deep whole-body hypothermia than in those treated with mild whole-body hypothermia (#P < 0.05, by Tukey test). Regional and whole-body normothermia resulted in a similar increase in OX-42 levels (P > 0.05, by two-way ANOVA). Whole-body hypothermia, either mild or deep, contributed to lower OX-42 levels than the corresponding regional hypothermia (P < 0.05, by two-way ANOVA). β-actin was used as a loading control. Error bars  represent mean ± SD; n = 5 rats per treatment.

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