Fig. 6. Photomicrographs showing glial fibrillary acidic protein (GFAP) immunoreactivity in the ipsilateral cuneate nucleus (CN) on day 7 days after chronic constriction injury (CCI) in rats that received regional hypothermic treatment. Little GFAP immunoreactivity was observed in the CN of sham-operated rats treated with regional normothermia (A ), mild regional hypothermia (B ), or deep regional hypothermia (C ). In preinjury and 5 h postinjury groups, GFAP immunoreactivity in the CN was decreased in CCI rats that received mild (E, H ) or deep regional hypothermia (F, I ) compared with those that received regional normothermia (D, G ). In 1 day, 3 days, and 5 days postinjury groups, intense GFAP immunoreactivity was observed in the CN of CCI rats treated with regional normothermia (J, M, P ), mild regional hypothermia (K, N, Q ), or deep regional hypothermia (L, O, R ). Bar = 100 μm.