Fig. 2. Immunofluorescence microscopy of glycine transporter 1 (GlyT1) expression in rat brain cortex coronal cryosections. (A ) GlyT1-related signals were found in the most superficial areas of the cortex at the parietal lobe, predominantly in cells that stained positive for glial fibrillary acidic protein, a specific marker for astroglial cells. (B ) Less superficial, most GlyT1-related fluorescence was found in cells that costained positive for neuron-specific nuclear protein. Note that digital overlays (Merge) demonstrate the colocalization of astrocytes and neurons with GlyT1. Nuclei were stained using DAPI. Similar results from spinal cord cryosections can be found in Supplemental Digital Content 1, https://links.lww.com/ALN/A790. GlyT1 = glycine transporter 1; NeuN = neuron-specific nuclear protein.