Fig. 4. MOR1 Overexpression increases proliferation, migration, and invasion and enhances sensitivity to Akt and mTOR inhibitors in human H358 non-small cell lung cancer cells. (A–C ) Graphic representations of the percent proliferation (A ), migration (B ), or invasion (C ) of stable vector control (VC) and μ-opioid receptor 1 (MOR1, the most abundant MOR transcript that consists of exons 1, 2, 3, and 434) overexpressing (O/E) H358 cells either untreated (control) or treated with 1 μM Akt Inhibitor XIII, 10 nM temsirolimus (mTOR complex 1 inhibitor), or 100 nM methylnaltrexone (MNTX, peripheral MOR antagonist) as described in the Methods section. Proliferation, migration, or invasion of untreated vector control H358 cells was set at 100%. There is a statistically significant difference (P < 0.05) in proliferation, migration, and invasion between basal VC versus  MOR1 O/E H358 cells. The asterisks  indicate a statistically significant difference (P < 0.05) between treatment and corresponding control groups.