Fig. 2. Effects of Ly6G/Gr-1 antibody on pain and inflammation after hind paw incision. (A ) Mechanical allodynia was measured in the neutrophil-depleted (AGr1 Ab) and control (C Ab) mice using calibrated von Frey filaments before and at different time-points after incision (n = 6 per group). (B ) Paw withdrawal latencies to heat stimuli were measured in both antibody-treated groups using the Hargreaves method (n = 7 per group). (C ) Measurement of paw edema using a laser assay in the antibody-treated mice (n = 7 per group). Mean ± SEM values of each group were analyzed by two-way ANOVA with post hoc  Bonferroni correction comparing treatment groups at each time-point. **P < 0.01, ***P < 0.001. Ab = antibody; AGr1 = anti-Gr-1; C = control; INC = incision.