Fig. 6. Postischemic treatment of intralipid and cyclosporine-A decreases cardiac reactive oxygen species generation as well as mitochondrial superoxide production. (A ) Representative dihidroethidium staining of transverse heart sections subjected to 20 min of global ischemia followed by 5 min reperfusion with phosphate-buffered saline (control group, black ), 1% intralipid (red ), and 1.5 μM cyclosporine-A (blue ). (B ) Fluorescence quantification of dihydroethidium staining: average intensity represents area · fluorescence intensity and is normalized to control (**P < 0.001 vs. control, n = 3). (C ) Superoxide production in isolated mitochondria using electron spin resonance in control (black ), 1% intralipid (red ), and 1.5 μM cyclosporine-A (blue ). **P = 0.005 intralipid versus control; *P = 0.041 cyclosporine-A versus control (n = 4). CsA = cyclosporine-A; CTRL = control; DHE = dihydroethidium; ILP = intralipid.