Fig. 6. Effect of chloroprocaine on the phosphorylation status of intercellular adhesion molecule-1 (ICAM-1) in NCI-H838 lung cancer cells. (A ) (i) Representative Western blot of NCI-H838 lung cancer cell ICAM-1, phosphorylated at tyrosine 512 (pY512 ICAM-1, row 1) and total ICAM-1 (row 2) after treatment with either tumor necrosis factor (TNF)-α (20 ng/ml) or different concentrations of chloroprocaine (1 nM, 1 μM, 10 μM, 100 μM) for 20 min. (ii) Representative blot of NCI-H838 cell pY512 ICAM-1 (row 1) and total ICAM-1 (row 2) after treatment with TNF-α (20 ng/ml) in the presence or absence of different concentrations of chloroprocaine (1 nM, 1 μM, 10 μM, 100 μM) for 20 min. (B ) Relative density of the Western blot bands of pY512 ICAM-1 normalized to the densitometry values of total ICAM-1 compared with control (= 1.0, dashed line) in the absence of TNF-α. Data from three independent experiments shown as scatter plot (n = 3). Horizontal line indicates mean for each group. (C ) Relative density of the Western blot bands of pY512 ICAM-1 normalized to the densitometry values of total ICAM-1 after coincubation of lidocaine with TNF-α (20 ng/ml) compared with TNF-α alone (= 1.0, dashed line). Data from three independent experiments shown as scatter plot (n = 3). Horizontal line indicates mean for each group.