Fig. 1. High-fat diet–fed mice exhibit endothelial dysfunction. Endothelial function was measured by the vasorelaxation response induced by acetylcholine (10−9–10−5M) in phenylephrine (10−5M) precontracted second-order mesenteric arteries. Standard diet, wild-type (WT) mice fed a standard diet (n = 7); High-fat diet, WT mice fed a high-fat diet for 4–6 weeks (n = 8). Data are expressed as mean ± SD. P = 0.01 by two-way analysis of variance with repeated measures.