Fig. 2.
γ-Aminobutyric acid type-A (GABAA) receptor in A549 cells displays unique functional features. (A) Patch clamp recordings showed that GABA (0.1–100 μm) evoked transmembrane currents in A549 cells in a dose-dependent manner (A-1), with EC50 of 2.45 ± 0.28 μm and ECMax of 32.66 ± 6.32 μm (A-2). (B) The current induced by 30 μm was largely inhibited by 100 μm bicuculline (B-1 and B-2; control: 80 ± 7.3 pA, n = 7; antagonist: 27 ± 4.1 pA, n = 7; **P < 0.01) and were almost fully blocked by 50 μm picrotoxin (B-2; control: 84 ± 11.0 pA, n = 5; antagonist: 3.3 ± 6.7 pA, n = 5; ***P < 0.001).