Fig. 3.
Rats that were anesthetized with sevoflurane (but not isoflurane) on postnatal days 4 and 5 (P4 and P5), spent significantly increased time in immobile state; an effect that was alleviated by pretreatment with bumetanide. A, Illustration of the experimental protocols. B, The effect of sevoflurane. Histogram showing time spent in immobile state by three treatment groups: (1) control (n = 19), saline + sevoflurane (n = 13), and bumetanide + sevoflurane (n = 10). * P < 0.05 versus control. C, The effect of isoflurane. Histogram showing time spent in immobile state in three experimental groups: Rats in the two treatment groups were exposed to isoflurane anesthesia after they were pretreated with either bumetanide (5 µmol/kg, intraperitoneally, n = 12 per), or equal volume of saline (n = 12). Rats in the control group (n = 19) did not undergo anesthesia at P4. All nonanesthetized rats tested in spontaneous behavior experiments were combined in the control groups.