Fig. 3.
Bone fracture exacerbates the recruitment of activated macrophages. A, Representative images of CD68 antibody stained section. A1, A low magnified picture showing the border zone (B.Z.) and the periinfarct region outside (P.I.) and inside the border (core). CD68+ cells formed a band just outside the infarct border. scale bar: 100 μm A2 and A3 are representative pictures taken in the periinfarct region outside the infarct border of mice with stroke (A2) or stroke plus bone fracture mice (A3). Scale bar: 50 μm. B, Bar graph shows the quantification of CD68+ cells in the periinfarct region (n = 7, * P < 0.001). C, Correlation between the numbers of CD68+ cells and terminal deoxynucleotidyl transferase deoxyuridine triphosphate nick end-labeling (TUNEL)+ neurons (r2 = 0.70, P < 0.001). DAPI = 4’,6-diamidino-2-phenylindole.