Fig. 5.
MSC therapy enhances the resolution of structural lung injury after VILI. MSC therapy enhanced resolution of histologic injury as evidenced by decreased alveolar lung tissue (A) and increased alveolar airspace fraction (B). Representative photomicrographs of lung from a vehicle-treated (C), and IT MSC-treated (D) animal demonstrate greater resolution of lung injury with MSCs at 48 h (n = 8 animals per group). Scale bar is 200 µm. CM = conditioned medium; IV = intravenous; IT = intratracheal; MSC = mesenchymal stromal cell; No Therapy = no treatment given; Vehicle = treatment with vehicle alone; VILI = ventilation induced lung injury. *Significantly (P < 0.05) different from Vehicle, fibroblast, and No therapy groups.