Estimation of ED50 of emulsified isoflurane for producing loss of consciousness (LOC) and immobility. (A) Circles indicate the percentage of volunteers in whom LOC was observed. LOC was defined as failure to follow loud verbal command to open his or her eyes. The solid line shows the result of probit analysis and the dashed lines indicate the 95% CIs of the best-fit line. (B) Circles indicate the percentage of volunteers in whom immobility to standard electrical stimulation (50 mA, 0.2 ms) was observed. Prevention of purposeful movement in unstimulated extremities to this electrical stimulus was regarded as immobility. The solid line shows the result of probit analysis and the dashed lines indicate the 95% CIs of the best-fit line. ED50 = median effective dose; PM = Prevention of purposeful Movement in unstimulated extremities.
